Assessment of their effect on growth promotion and phosphorous uptake of mung bean vigna radiata l. Phosphatesolubilizing bacteriaassisted phytoremediation. Maize endophytic bacteria as mineral phosphate solubilizers. Isolation and characterization of a phosphatesolubilizing. Int j nutr pharmacol neurol dis serial online 20 cited 2019 oct 9. Phosphatesolubilizing bacteria play a vital role in p solubilization by producing organic acids. Application of phosphatesolubilizing bacteria psb has been shown to promote plant growth and development in the. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. In soil phosphate solubilizing bacteria constitute 1 to 50%,while phosphate solubilizing fungi are 0. Culturable total and beneficial microbial occurrences in. Additionally, soils contain low molecular weight organic acids with one or more carboxylic groups and some of these acids like citrate, oxalate, acetate, malate, isocitrate, and tartrate.
This study examined bioremediation of crude oil polluted soil ps stimulated with nitrogenfixing bacteria nfb and phosphatesolubilizing bacteria psb. Among 10 strains screened for zn solubilisation, p29, p33, and b40 produced 22. The optimum conditions for phosphate solubilization under in vitro condition were found 35 c at. Total petroleum hydrocarbons tphs were monitored for 4 weeks. Phosphate solubilizing bacteria associated with vegetables roots in different ecologies article pdf available in pakistan journal of botany 45. Phosphate solubilizing bacteria psb are the bacteria that possess the capability to change the insoluble form of phosphorus into soluble one.
Phosphate solubilizing bacteria play a vital role in p solubilization by producing organic acids. Phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement. Soil inoculation with phosphate solubilizing bacteria. Substantial quantity of applied inorganic zinc in soil is converted into unavailable form.
Isolation and characterization of phosphatesolubilizing bacteria from rhizosphere soil. Isolation and characterization of phosphate solubilizing. The pgpr xsb375 enhances seed germination, early emergence, plant vigor, root and shoot growth, and higher crop yield. Five highly efficient psb strains, h22, y11, y14, y34, and s32, were screened and isolated from an alfalfa rhizosphere in heavy metalcontaminated reclamation area in shanxi province, china. Phosphate solubilizing potentiality of the microorganisms capable of utilizing aluminium phosphate as a sole phosphate source. Phosphate solubilizing bacteria and their role in plant growth promotion article pdf available in biotechnology advances 1745. Isolation and characterization of phosphatesolubilizing bacterial.
These isolates were identified using light microscopy and 16s rrna gene. Pdf forty two isolates were recovered as phosphorus solubilizing bacteria psb from rhizosphere of healthy plants of pea, spinach, ladys finger. Isolation of phosphatesolubilizing bacteria and their ability to solubilize calcium phosphate from the rhizosphere soils tomato, jasmine, onion, and maize, the total of 12 bacterial species of 32 samples were found enriched on pikovskayas agar plates. The bacteria and fungi convert nonlabile p through either inorganic psolubilizing or organic p mineralization processes. The native populations of phosphatesolubilizing bacteria and fungi were studied in. The study of inorganic insoluble phosphate solubilization. Phosphate solubilizing bacteria bioline international. Phosphate solubilizing microbes have been reported for plant growth promoting and enhancing yield kapoor, mishra, and kuhkreja 1989.
Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. The pgpr is capable to solubilize phosphate that enhance plant available phosphorous. Phosphate solubilizing bacteria psb population and phosphate solubilizing activity a total of 12 phosphate solubilizing bacterial isolates were found in this study. It was observed that a large proportion of bacteria isolated from garden soil can exert phosphate solubilizing activity. Phosphate solubilizing bacteria can increase soil p availability. Few studies, however, have focused on communities of. Phosphatesolubilizing activity of aerobic methylobacteria. Isolation and characterization of phosphatesolubilizing. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting. Isolation and classification of nitrogen fixing and.
Comparative study of phosphate solubilizing bacteria from. Effect on plant growth and yield article pdf available december 20 with 541 reads how we measure reads. Isolation and screening of phosphate solubilizing bacteria. However, direct use of rock phosphate is not recommended because of its low availability, so it is necessary to impro ve it. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. Similar to nitrogen fixing bacteria, the psb population was higher in the rhizosphere compared to the. In this study, highthroughput isolation hti was used to identify n fixing andor p solubilizing bacteria. Page 54 introduction after nitrogen, phosphorus is the most important element limiting plant. Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment. This article is published with open access at abstract heavy metal pollution of soils is of great concern. Phosphate solubilizing bacteria can protect plants from deleterious.
Influence of nutritional and environmental conditions. Pdf selection and evaluation of phosphatesolubilizing. The bacteria and fungi convert nonlabile p through either inorganic p solubilizing or organic p mineralization processes. Effect of phosphate solubilising bacteria bacillus psb24. Isolation of phosphate solubilizing bacteria and fungi. Study of influence of phosphate dissolving microorganisms. Phosphatesolubilizing bacteria and fungi play an important role in p bioavailability and can be used to increase pue bolan et al. Inoculation of phosphate solubilizing micro organisms increase crop yield by 510 %. Phosphate solubilizing microbes plays an important role in plant nutrition crease pr reactivity the use of. It has been worldwide reported that by implementation of phosphate solubilizing bacteria inoculum increase growth and. Phosphate solubilizing bacteria research papers academia. Phosphate solubilizing activity is greater for fungi than bacteria. Isolation and characterization of phosphate solubilizing microbes from agricultural soil t. Selection and evaluation of phosphatesolubilizing bacteria from grapevine rhizospheres for use as biofertilizers article pdf available december 2016 with 1,169 reads how we measure reads.
The present invention relates to a composition useful for qualitative screening of phosphate solubilizing microorganism, and also relates to a synergistic composition for qualitative screening of phosphate solubilizing microorganisms, based upon visual observation. Pdf functional aspect of phosphatesolubilizing bacteria. The growth of methylobacteria on medium with methanol as the carbon and energy source and insoluble tricalcium phosphate as the phosphorus. Inoculation of maize with phosphate solubilizing bacteria. Comparative efficiency of five potash and phosphate solubilizing bacteria and their key enzymes useful for enhancing and improvement of soil fertility sajidmohammad a saiyad, dr. Phosphate solubilizing bacteria psb has ability to convert insoluble form of phosphorous to an available form. The search for life in space documentary where is the centre of the universe touch your heart 470 watching live now. Phosphate solubilizing bacteria, pseudomonas fluorescens, siderophore. Phosphatesolubilizing bacteriaassisted phytoremediation of metalliferous soils. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. View phosphate solubilizing bacteria research papers on academia. Effect of phosphate solubilising bacteria bacillus psb24 on growth of tomato plants.
Phosphate solubilizing bacteria psb play an important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments. In fact, the ability of heterotrophic bacteria to solubilize insoluble phosphate is well documented new et al. Among the bacteria able to solubilize phosphate, the genera. The present study focuses on the phosphatesolubilizing capacity of bacteria and fungi in rhizospheres soil samples. Several researchers have reported that inoculation with phosphatesolubilizing bacteria result in improved growth, yield, and p uptake in several crops hameeda et al. Application of potential phosphatesolubilizing bacteria. Pdf phosphate solubilizing bacteria associated with vegetables. The aim of this study was to isolate, characterize and use phosphate solubilizing bacteria to enhance the bioavailability of insoluble caphosphate for wheat plants. Phosphate solubilization by endophytic bacteria isolated. Phosphatesolubilizing bacteria psb is slowly emerging as important. Zinc solubilising bacteria are potential alternates for zinc supplement.
Phosphate solubilizing rhizobacteria as alternative of chemical. A biologically pure phosphate solubilizing plant growth promoting rhizobacteria pgpr xsb375 was isolated from canola rhizosphere and identified as bacillus firmus is provided herein. Isolation and characterization of a phosphate solubilizing heavy. The effects of psb on microbial activity and soil quality are not well known. Pdf phosphate solubilizing bacteria associated with. The integrated use of rock phosphate and psm add 2030 kg p2o5ha. Screening the effect of phosphate solubilizing bacteria on. Microorganisms play an important role in the availability of soil phosphate to the root system and enhance the mobilization of phosphate in soil richardson, 2001. Soil ph is commonly considered a dominant factor affecting the function of microbiota. Effect of immobilized phosphate solubilizing bacteria on. Comparative efficiency of five potash and phosphate.
Glycineglomusphosphate solubilizing bacteria interactions lead to fertilizer phosphorus economy in soybean in a himalayan acid alfisol. Functional aspect of phosphatesolubilizing bacteria. A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment and fresh water environment like lake ecosystem. Inoculant carriers, rhizobacteria, bioencapsulation, rock phosphate, solubilization. Pdf phosphate solubilizing bacteria and their role in.
This study was aimed to isolate phosphate solubilizing bacteria from sugarcane waste compost. For this purpose, 15 phosphorus solubilizing bacteria psb were isolated from wheat rhizospheric soils of peshawar and southern punjab region, pakistan. Phosphate solubilizing biofertilizers as a component of inm many heterotropjic bacteria and fungi efficiently solubilize insoluble phosphate of soil and rock phosphates. Similarly, inoculation of maize and wheat plants with. Whereas, strains isolated from clinical samples and air showed decreased p solubilization. Characterization of diazotrophic phosphate solubilizing. The present work is comparative study on phosphate solubilizing bacteria isolated from rhizosphere of rice plant and from nonrhizospheric soil bulk soil of the paddy field during flowering and harvest stage. Zinc zn is one of the essential micronutrients required for optimum plant growth. Table 2 3 description of the source and the numbers of putative nitrogen fxing bacteria and phosphate solubilizing bacteria 50 table 2 4 enumeration of putativen fixing ang p. Study of influence of phosphate dissolving microorganisms on yield and. Responses to soil ph gradients of inorganic phosphate.
Patel and parmer, 20 reported that 11 isolates were found good phosphate solubilizer from rhizosphere soil of sunflower field at surat, bharuch district of gujarat. Us6638730b2 composition for qualitative screening of. The goal of this research lies in the identification of beneficial bacteria capable of fixing nitrogen and solubilizing phosphate. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Phenotypic and genotypic characterization of phosphate solubilizing. Journal of soil science and plant nutrition, 20, 1, 110. Occurrence, mechanisms and their role in crop production ahmad ali khan 1, ghulam jilani 1, mohammad saleem akhtar 1, syed muhammad saqlan naqvi 2, mohammad rasheed 3 abstract plants acquire phosphorus from soil solution as phosphate anion. Prospective zinc solubilising bacteria for enhanced. The use of phosphate solubilizing bacteria as inoculants simultaneously increases phosphorus uptake by the plant. The study of inorganic insoluble phosphate solubilization and.